With the development of technology, Cytiva chromatographic fillers have moved from the old generation of fillers such as Sephadex/Sepharose into the era of modern fillers with new substrates such as MabSelect/Capto. The advantages of more efficient and faster also achieve the purpose of "reducing cost and increasing efficiency" for a wide range of chromatographic purification operations.
Higher flow rate
As shown in the figure above, the diameter of the loading column is 1m. Under the condition of 20℃, the water flow rate at different loading heights is different. The red and blue curves respectively represent the actual performance of different base fillers under pressure limits. This is due to the update of the new base, Capto packing has higher rigidity and lower back pressure than Sepharose packing, so Capto packing with higher column bed height can still achieve very good flow rates, and the more selective column height provides a highly flexible process operation window for each experimenter.
Higher dynamic load
As shown in the figure above, the surface of the Capto filler has a linear dextran extension arm, which has nearly twice the dynamic load as Sepharose, and the required retention time is greatly reduced. The increased dynamic load capacity means that each Capto chromatography purification is significantly less than the amount of Sepharose filler used, and the cohhesponding solution amount is also reduced exponentially, resulting in a reduction in balancing and elution time, which will significantly improve the efficiency of the purification and separation cycle.
High resolution and high flow rate
The Capto ImpRes series combines the high flow rate characteristics of the Capto series with the high resolution performance of the Sepharose HP series. As can be seen from the above figure, Capto ImpRes has higher resolution in chromatographic purification with the same retention time; While maintaining the same resolution separation effect, Capto ImpRes can be achieved faster than the Sepharose HP series. This is also an efficient and highly protective purification design concept for precious protein samples in chromatographic separation.